PFT Technology:
 

Quadruple antibodies against porcine epidemic diarrhea

, transmissible gastroenteritis, rotavirus and pseudorabies disease

 
R & D Background
Since 2010，the piglet epidemic diarrhea is outbreak in pig enterprises in Southern China and spread to Northern China within 3 months. The incidence rate was 50-80% while the mortality rate reached to 70-80% in winter time. The diseases mainly occurred in the postpartum piglets and induced higher death in the piglets. Due to the physical weakness, the sick piglets are easy to be infected with other viruses, such as transmissible gastroenteritis virus, rotavirus, reproductive and respiratory syndrome virus and circovirus. The Porcine epidemic diarrhea virus (PEDV), was first described in Europe in the 1970s and since then has spread over many Asian and American countries, where it recently led to devastating effects on swine health and pork industry. At present, immunization is an effective way to control the epidemic diarrhea of piglets and vaccines are used for sow immunization. However, vaccine for piglets is unavailable in world. Moreover, the inactivated vaccine and live vaccine are unable to provide a full protection from infection of viruses due to the occurrence of the new virulent strains.
Generally, highly immunity of piglets can be obtained the specific antibody from sow. Particularly,28 hours after piglet birth, immunoglobulin can be absorbed through the intestinal mucosa and then enters blood circulation. In this sense, piglets are able to get the direct protection from sow’ milk. On the contrary, the piglets fail to get high levels of antibody from colostrum, piglets will be at high risk of the infection.
In recent years, the abuse of the antibiotics for growth and disease prevention has been under severe scrutiny in livestock industries. The use and misuse of in-feed antibiotics has led to potential risks for human health, including drug residues in animal products and bacterial resistance. On other hand, chicken egg yolk antibodies (IgY) are alternative approaches to antibiotics. More important, oral administration of IgY possesses many advantages over mammalian IgG, such as effectiveness, safety, lower cost and rich resources. In our pioneer study, the applications of IgY in the control of enteric infections of viral origin are optimistically confirmed, such as rotavirus, porcine transmissible gastroenteritis virus, and porcine epidemic diarrhea virus. In the past two decades, a variety of materials have been tested as effective alternatives to antibiotics for maintaining swine health and performance. The most widely choices include enzymes, organic acids, probiotics and prebiotics and herbal extracts. However, these productions are difficult to meet the requirement between the growth improvement and diseases control.

IgY advantages
In addition to good protection, IgY antibody originated from hen eggs has a number of advantages over production methods using mammals.

• Oral administration to piglets without any stronger stress in comparison with the injection.
• IgY purified from hen eggs has no risks of spread of pathogens compared to the IgG from porcine serum.
• In contrast to antibiotics, IgY is environmentally-friendly product elicits no undesirable side effects and toxic residues.
• IgY does not activate mammalian complement and also does not interact with mammalian Fc and complement receptors. Also, IgY does not bind to protein A, protein G or rheumatoid factor. These differences provide the diverse advantages of IgY technology as compared to those of the antibiotic therapy.
• IgY possesses many advantages over mammalian IgG such as cost-effectiveness, convenience and high yield.

Preliminary clinical trials
Oral administration with 5 ml antibodies per newborn piglet before sucking activities. In severely infected pig farms, the diseased piglets are recommended to receive 5ml antibodies again and the antibody will protect piglets until weaning. Clinical trials showed that the average protection rate amounts to 90% survival rate post administration with the antibody and the average live piglets are more than 11 in numbers. On contrary, the survival rate is 25.9%-66.0% and the live litter size are arranged from 3.1 to 7.6 baby piglets.

Application instruction of super-antibody against piglet diarrhea

[Active substances]
IgY antibodies against porcine epidemic diarrhea virus, infectious gastroenteritis virus, rotavirus and pseudorabies virus; protective factor of the intestinal mucosal.

[Properties]
Pale yellow liquid，pH 5.0~6.0.

[Target species]
Piglets

[Application]
Used for the newborn piglet diarrhea, fever and anorexia caused by porcine epidemic diarrhea, transmissible gastroenteritis, rotavirus and pseudorabies virus.

[Method and application route]
Oral spray or drench within 24 hours after birth. As for severely infected piglets, additional 5ml is recommended for piglets aged 5-day old and the protection will last until weaning.

[Withdrawal period] N/B

[Storage] Store in a refrigerator (2-8^oC). Do not freeze! Protect from light. Once opened, use immediately

[Package]  5L/barrel，20L/box   
 
[Expiry days] 12 months